Pinhole Radius

Pinholes are used in confocal, spinning disk, STED and STED 3D microscopy to get rid of out-of-focus light. Since there’s no pinhole in widefield and two photon microscopes HRM skips this parameter in those cases.

PinholeRadiusScreenshot

Note

Notice that HRM needs the value of the pinhole backprojected on the specimen.

Further help on how to calculate the backprojected pinhole radius can be found on PinholeCalculatorLink.

Pinhole Spacing

Spinning disk microscopy uses more than one pinhole to speed up image acquisition. The backprojected distance between these pinholes also plays a role in image deconvolution. HRM skips this parameter for all other microscope types.

PinholeSpacingScreenshot

Click on PinholeCalculatorLink for further help on the calculation of the backprojected value.