Voxel Size

The voxel size or sampling size is a very important parameter in image deconvolution.

Note

According to the Nyquist criterion its value should not be larger than half the optical resolution of the imaging system.

HRM offers several utilities to help you set up this parameter correctly.

VoxelSizeScreenshot

Ideally, the optimal voxel sizes are calculated before acquiring the images. By doing so, one can ensure that the images are compliant with the Nyquist criterion. Click on NyquistCalculatorLink to determine how large the voxel sizes should be. By using these values, there will be no lost information during the image acquisition and the deconvolution will do a nice job in restoring your images.

If the optimal voxel sizes have not been determined before the acquisition stage one can calculate the resulting sizes in widefield and spinning disk microscopy by using CCDCalculatorLink. Here the voxel sizes are calculated from the CCD camera element, the objective magnification, etc., although no optimal voxel size can be guaranteed.

Note

Make sure to set a voxel size consistent with the Nyquist criterion. Undersampled images will often show artifacts after deconvolution.