PSF TypeΒΆ
To perform image deconvolution a Point Spread Function (PSF) is needed.
The Huygens software can compute a theoretical PSF from the image parameters used during deconvolution or it can use a measured PSF.
Depending on the user choice HRM will, at a later stage, ask for:
- the path to the file containing the PSF if you selected Measured,
- the settings to correct for spherical aberration if you selected Theoretical and there is a refractive index mismatch.
In most cases a theoretical PSF works fine.