Launch the job (5/5)

In this last step several summaries display all the chosen parameters.

Also, the output format for the result images can be selected at the top of the page:

• ICS, ICS2 and HDF5: support multi-channel images, 32 bit dynamic range (preserve all image details) and all the microscopic metadata parameters. Furthermore, they support high compression levels. Since deconvolved images often contain background regions with near 0 intensities the compression algorithms usualy manage to greatly reduce the size of the images. For all these reasons these are the formats recommended for further work with Huygens.
• TIFF: this format can be used for analysis such as counting or segmentation. For quantification one needs to scale the TIFF image back to its original dynamic range. The corresponding scaling factors are reported by HRM and can be found in Parameter summaries.
• IMS, OME-XML, R3D: formats offered for compatibility with other programs.

Next, review all the selected parameters before submitting the job to the server. It’s possible to go back to the image, restoration, and analysis template editors by clicking on the corresponding links.

Click on to change the image parameters.

Click on to change the restoration parameters.

Click on to change the analysis parameters.

To change the image selection click on .

Finally submit the job by pressing .