Advanced deconvolution in HRM

• The Home panel
• Launch a job
  • Select images (1/5)
  • The Image Parameters (2/5)
    • Number of channels
    • PSF Type
    • Microscope type
    • Import metadata
    • Numerical aperture
    • Wavelengths
    • Objective Type
    • Sample medium
    • Voxel Size
    • Using a measured PSF
    • Pinhole Radius
    • Pinhole Spacing
    • STED parameters
    • Spherical Aberration correction
  • The Restoration Parameters (3/5)
    • Deconvolution algorithm
    • Signal to Noise ratio
    • Crop image
    • Background mode
    • Stopping criteria
    • Stabilization along Z
  • Analysis parameters (4/5)
    • Colocalization
    • Channels
    • Colocalization coefficients
    • Threshold
    • Colocalization map
  • Launch the job (5/5)
• The Queue status
• Results: detailed view
  • Comparing MIPs
  • Comparing SFPs
  • Comparing Z Slices
  • Comparing T frames
  • Z, T and T-SFP movies
  • Colocalization
  • Parameter summaries
  • Huygens log
• Tips & Tricks

This section explains in detail how to create and launch deconvolution jobs in HRM. It also describes how to inspect deconvolution results and how to view user statistics.

For the sake of clarity, it is recommended to emulate the explanations here in an actual HRM installation. For instructions on how to request an HRM account please see Create an account in HRM.

With an HRM account deconvolution and analysis jobs can be run remotely, in batch mode, with just a few clicks.